Sunday, December 29, 2019

Essay about Hawthornes Young Goodman Brown †Point of View

In Nathaniel Hawthorne’s â€Å"Young Goodman Brown† how does the author present the characters, dialogue, actions, setting and events which comprise the narrative in this short story? This essay will answer these questions. R. W. B. Lewis in â€Å"The Return into Time: Hawthorne† states that â€Å"there is always more to the world in which Hawthorne’s characters move than any one of them can see at a glance† (77). In Hawthorne’s â€Å"Young Goodman Brown† this fact is especially true since the main character, Goodman Brown, is a naà ¯ve hero and since the narrator tells much of the story through the limited point of view of the protagonist. In this story the author uses a third-person narrator, who uses proper names and third-person pronouns to†¦show more content†¦. . . The young man sat a few moments by the road-side, applauding himself greatly, and thinking with how clear a conscience he should meet the minister, in his morning-walk, nor shrink from the eye of good old Deacon Gookin. And what calm sleep would be his, that very night, which was to have been spent so wickedly, but purely and sweetly now, in the arms of Faith! Amidst these pleasant and praiseworthy meditations, Goodman Brown heard the tramp of horses along the road, and deemed it advisable to conceal himself within the verge of the forest, conscious of the guilty purpose that had brought him thither, though now so happily turned from it. . . .Young Goodman Brown caught hold of a tree, for support, being ready to sink down on the ground, faint and overburthened with the heavy sickness of his heart. He looked up to the sky, doubting whether there really was a Heaven above him. . . . He knew the tune; it was a familiar one in the choir of the village meetinghouse. . . . But, where is Faith? thought Goodman Brown; and, as hope came into his heart, he trembled. . . . At the word, Goodman Brown stepped forth from the shadow of the trees,Show MoreRelated Point of View of Hawthornes Young Goodman Brown Essay1512 Words   |  7 PagesPoint of View of â€Å"Young Goodman Brown†Ã‚  Ã‚  Ã‚  Ã‚         Point of view is â€Å"one of the most prominent and persistent concerns in modern treatments of the art of prose fiction† (Abrams 231). This essay will treat of how the story is told in Nathaniel Hawthorne’s â€Å"Young Goodman Brown,† what type of narrator tells it, and through whose perception the reader receives the tale – in other words, the point of view of this short story (Axelrod 336).    In this story the mode or point of view by whichRead MoreYoung Goodman Brown from a Moral Standpoint1352 Words   |  6 PagesHawthorne discovered that his ancestors were founders and Puritan leaders of the Salem witch trials. Shortly after this tragic finding, he wrote â€Å"Young Goodman Brown,† a tale that is considered one of the greatest in American literature. Analyzing Nathaniel Hawthorne’s work from a moral perspective can help illuminate his short story: â€Å"Young Goodman Brown.† Hawthorne was both prideful and embarrassed in the actions of his ancestors. According to Jacqueline Shoemaker, Hawthorne felt pride in seeingRead MoreAnalysis Of Nathaniel Hawthorne s The Great Gatsby 1416 Words   |  6 PagesEdgar Allan Poe, born in the year 1809, in Boston, Massachusetts, was also a writer in Dark Romanticism. An orphan at a young age, Poe was going through a tough childhood. He took in gambling in his college years, and enlisted in the army. Struggling through poverty, he managed to win a contest with his short story, and he started devoting his life to writing. He married his young cousin, Virginia, who was 13 years old in the year 1836. Dark Romanticism is a genre branched off of Romanticism, whereasRead MoreAnalysis Of Hawthorne s The Man s Fall 1438 Words   |  6 PagesCalvinistic, and ancient beliefs that form the basis of Hawthorne’s work (Hawthorne 392). Besides, the researcher notes that Hawthorne’s life is authentic although fictional . There is a psychological aspect of the story in that the man’s fall is unavoidable. The narration contains metaphoric and literal journey of the newlywed male character. However, he is making the treacherous journey with the devil himself therefore creating a spiritual crisis. Goodman Brown’s decision is ambiguous to his audience becauseRead MoreNathaniel Hawthorne s Young Goodman Brown1065 Words   |  5 PagesWhen it comes to the topic of Nathaniel Hawthorne’s Young Goodman Brown, most of us will readily agree that duplicity is a major theme in the piece, or the idea of different versions of reality. Where this agreement usually ends, however, is on the question of whether Hawthorne is implying that man is inherently evil. Whereas some are convinced that Young Goodman Brown was good until tainted by the Devil, others maintain that he was evil from the beginning and was completely aware of the evil heRead MoreShort Story Analysis: Young Goodman Brown Essay1 115 Words   |  5 Pagesmore intriguing than Nathaniel Hawthorne. Hawthorne’s ability to weave stories through the use of complex language and early puritan society narratives has long been a topic of study amongst scholars and young adults, alike. â€Å"Young Goodman Brown† explores the idea of good vs. evil and draws many parallels to the life of Nathaniel Hawthorne. It is often debated whether man is born innately good or evil. In â€Å"Young Goodman Brown† it is possible to see Hawthorne’s stance on this. However, before delvingRead More Nathaniel Hawthornes Young Goodman Browns Apocalypse Essay1006 Words   |  5 PagesNathaniel Hawthornes Young Goodman Browns Apocalypse      Ã‚  Ã‚  Ã‚  Ã‚   Most criticism and reflection of Nathaniel Hawthornes Young Goodman Brown centers on a good versus evil theme. Critics also debate interpretations of the main characters consciousness; is Brown awake or dreaming.   What is certain is that he lives and dies in pain because his belief in his righteousness isolates him from his community.   It is also certain that Hawthornes interpretation of Browns mid-lifeRead MoreNathaniel Hawthorne s Young Goodman Brown1492 Words   |  6 PagesIn Nathaniel Hawthorne’s short story of Young Goodman Brown, the author uses symbolism and allegories in order to showcase the Puritan faith as well as man’s conflict between good and evil. This analysis will breakdown the techniques that the author uses to critique the puritan society, and to show the difference between how people appear to be in society and the true colors that they are hidden inside of them. There has been a lot of great authors in our time, but none more interesting than NathanielRead MoreYoung Goodman Brown, by Nathaniel Hawthorne865 Words   |  4 Pagesequally, if not more, serpent-like staff carrying dark companion. This respectable man is Young Goodman Brown, as portrayed in Nathaniel Hawthorne’s short story â€Å"Young Goodman Brown†. The forest is only a small part of the setting, as this also takes place in a village in Salem Massachusetts and surrounding area the year 1692. The mood is heavy with superstition, confusion, doubt, betrayal, and shallowness. Goodman Brown sets the exposition as he parts with his sweet, pink-capped wife, Faith, to leave atRead MorePoint of View in Young Goodman Brown Essay818 Words   |  4 PagesIn Nathaniel Hawthornes Young Goodman Brown, the story is told from a limited omniscient third-person narrator point of view. Limited omniscient third-person narrator means that the focal point of the story is limited to one character. This technique works well with the story because it allows the narrator to portray what Goodman Brown is doing, and also allows him to assess and remark on Goodman Browns doings throughout the story. Sometimes this method is used to convey the beliefs of the author

Thursday, December 26, 2019

Mobile Marketing And Advertising Trends - 1004 Words

MIS 204 Di Han 29/04/2016 Mobile Marketing and Advertising Trends Introduction: With the last ten years, Mobile advertising is a rapidly increasing part affording brands, business, and marketers more opportunities to connect with consumers, which overtop traditional and digital media immediately on their mobile phones. Nowadays, cell phones can not only for receiving and making calls but also can text messaging, content downloads and the Mobile Web. All those media channels carry advertising and content. When we say mobile advertising, not every channel is created equal. So as to reach the target customers, companies should focus on the apps and services when and where consumers spend their most of time. Which means they can use those†¦show more content†¦With one year later, Admob, a mobile advertising company set up.† In 2011, Mobile Services revenue exceeded 1$ Trillion for the first time†. (â€Å"History†) All those data told us, the market for the mobile advertising is not only growing but exploding. The Current Market for Mobile Marketing Advertising There is no evade from advertising and promotions when people are mobile, which becomes pervasively in our life.† According to the Interactive Advertising Bureau, the mobile advertising industry experienced a worldwide growth of 65 percent in 2014. From $19.3 billion in 2013, the industry achieved a net worth of an eye-popping$31.9 billion in 2014†. (â€Å"What†) The greatest part is that the sector is nowhere near slowing down. Mobile phones have gone through a vast evolution since 1983. It is also a core makeup of the modern consumers’ life. Nevertheless, the company still not sure whether revenues would follow users to the mobile market and also worried about the ability of the enterprise which can monetize mobile devices to make extraordinary profit successfully. There are some great examples: â€Å"According to the Twitter earnings report for the fourth quarter of 2014, their company makes advertising revenue for $432 million. Mobile advertising revenues accounted for a stunning 88 percentage of the total income, which has an over 85 percent increasing reported for the third quarter.† (â€Å"Twitter†) Facebook also not far behind, â€Å"according to their second

Friday, December 20, 2019

The Benefits of Obamacare Essay - 969 Words

Because millions have been able to free ride the health care system Obama emplaced a new health reform changing thousands of lives, making healthcare affordable for all people and giving citizens who are already covered new or more benefits. Healthcare has always been a pricey yet necessary asset to life. In the 1920s the middle class seemed to have struggled with the growing cost of medical visits. In the late 1920s a Dallas hospital started to offer joint affordable healthcare for only $.50 a month. Soon other states started to use the same method. The US began to identify the process as the Blue Cross. In that same year the stock market crashed and the Great Depression started. Essentially crushing everyones financial stability, the†¦show more content†¦When John Kennedy became president the American medical so you going to promote Medicare a health insurance for people with disabilities in stage diseases and people over 65. Hed advertised on the television, the radio, la rge mailings, and speaking tours, even churches began to involve themselves to support Medicare. Kennedy spoke to one large groups of people who traveled across the country to support the legislation. While Kennedy failed to pass Medicare the issue didnt die. Healthcare continue to advance and so did the price. When Lyndon Johnson went into office more than 60% of the voters supported Medicare (HNN). With large gains in both houses Johnson was swayed to push forward with the Medicare act in 1965 Congress passed Medicare and the Social Security act that included the health benefits and Medicaid which was provided to lower income Americans (HNN). In 1773 Richard Nixon won his presidency. The government came up with a reform involving private enterprise to promote the Health Maintenance Organization which were to require doctors toShow MoreRelatedThe Benefits Of Obamacare And No Insurance Company1239 Words   |  5 Pagesserious illness like Cancer. One things is for certain, health insurance is beneficial to everyone and everyone should have it. The President Barack Obama felt that everyone should have health care as well and so he created Obamacare. Health Insurance is a must have now that Obamacare is in effect and no insurance company can deny anyone for coverage, no matter their illness nor age. The UnitedHealth Group is a fortune 500 company and they serve over 85 million patients all over the world and that numberRead MoreBenefits Of Obamacare : Huy Gip2224 Words   |  9 Pages BENEFITS OF OBAMACARE Huy Gip University of Central Florida Abstract Health has become a primary concern in the United States of America. Bridging the gap between the rich and the poor in a purely capitalistic nation has proven to be quite a task. Languishing in poverty in a super power nation is even more demeaning in comparison to poverty stricken nation, which is expected. The various inputs that steer economic development work in tandem with the policies that have been signedRead MoreThe Many Benefits of ObamaCare Essay1768 Words   |  8 PagesAct created Medicare and Medicaid. Although this law has faced fierce opposition, the Affordable Care Act will help Americans lead healthier lifestyles, while increasing their financial stability. Under the Affordable Care Act, also known as ObamaCare, insurance companies are no longer allowed to discriminate against individuals with pre-existing health conditions. Uninsured children with pre-existing conditions, such as cancer or diabetes, are now able to access health insurance plans. NotRead More The Benefits of ObamaCare Are Greater Than the Costs Essay694 Words   |  3 Pagesless and less towards their employee’s healthcare which in turn has made insurance too costly for the average employee to continue the same amount of healthcare coverage. While companies have been lessening their contribution to employee health benefits insurances have continually raised their prices. In the recent years between 2001 and 2007 the cost of health care premiums has escalated almost 80% (Health Care Marketplace Project, 2008). To understand the dispute surrounding the constitutionalityRead MoreObamacare: The Future of Healthcare1143 Words   |  5 PagesAffordable Care Act or â€Å"Obamacare† is the law that will change health care forever. Obamacare will help Americans because it will give every American access to healthcare, lowers the healthcare costs while improving the quality, and will give patients new consumer protections. My first reason for supporting Obamacare is that there is now a variety of new consumer rights and protections for patients. Obamacare is the most munificent healthcare law in American history. Before Obamacare, patients could getRead MoreHealth Care Controversy958 Words   |  4 Pages Health Care Controversy Essay ObamaCare, also known as the Patient Protection and Affordable Care Act, is a Health care reform law that was signed on March 23, 2010. ObamaCare’s goal is to provide more Americans with affordable health care insurance. ObamaCare also hopes to improve the quality of healthcare and health insurance in America, regulate the healthcare industry, and reduce the cost of healthcare in the United States. ObamaCare is made up of ten titles; I: Quality, AffordableRead MoreObamacare : The Affordable Health Care Act1610 Words   |  7 Pagesknown as Obamacare. This new program has been so purely monumental, because government until then had not been directly involved with health care as much as they are. Benefits include lower costs, and thousands of uninsured individuals and families with health care. Downsides are more and higher taxes, and health insurance goes up. There has been much controversy centered around this topic, and many debates bring the good and bad points of Obama care. One significant fact about Obamacare, is thatRead MoreObamacare : The Patient Protection And Affordable Care Act1105 Words   |  5 PagesObamaCare, officially known os the the Patient Protection and Affordable Care Act, is a health care reform law signed in 2010 by President Barack Obama. Many of the law’s provisions are already in effect and the rest continue to roll out until 2022. Obama care offers patients ways to afford healthcare and get better treatment. The plan to put Obamacare into effect started in 2010 but was actually put into action in 2014. The Affordable Care Act also known as Obamacare increases the quality, accessibilityRead MoreThe Affordable Care Act ( Aca ) Or Obamacare?1655 Words   |  7 Pages The Pros and Cons of the Affordable Care Act (ACA) or Obamacare? Who Benefits and Who gets Hurt? Chanokporn Srisuwan, Student MBAA 605 Business, Government, and Society Mercy College School of Business- MBA Program August 04, 2016 Abstract The propose of this article is to present the advantages and disadvantages of the Affordable Care Act (ACA) or Obamacare which make all Americans insurable, by requiring insurers to accept all applicants at rates based onRead MoreEssay on ObamaCare and How It Greatly Affects Our Health Care System1644 Words   |  7 PagesObamaCare and How It Greatly Effects Our Health Care System Final Research Paper: ObamaCare and How It Greatly Effects Our Health Care System As the years go on, everything around us is constantly changing and becoming more advanced. The recent election of our president has and will force us to see some very big changes in the next few months. ObamaCare also known as the Affordable Care Act was set in place by President Obama in order to reduce health care costs and give affordable health insurance

Tuesday, December 17, 2019

The Rule Of The United States Constitution - 1483 Words

Change is Good Since the year of 1787, the United States Constitution has persisted as the highest law in our land. At the Constitutional Convention in 1787, delegates were determined to set the Articles of Confederation aside and devise a fresh concept. To ensure that we would have a government with rights and freedoms, the delegates created what was called the separation of powers. The reasoning behind the division of government was to ensure that no one branch could vanquish the other. As a result, three separate branches were created in the Constitution known as: the legislative, judicial and executive branches. Since the eighteenth century, America has changed significantly; and while we still uphold the same decrees that lie†¦show more content†¦Aside from the numerous responsibilities required of those in the Executive Branch, they also gain certain powers within their domain. In Article II of the constitution the Executive Branch is given the power to enforce laws, but the main power to enforce laws belongs to the President. The Presidential powers listed in the U.S. Constitution include: â€Å"being able to veto a proposal for a law, appoint federal posts, negotiate foreign treaties with other countries, appoint federal judges, grant pardons, and pass something called an executive order†. The President is also the commander-in-chief, chief executive, legislative leader, and chief diplomat. Besides all of the power that the President has when elected into office, how does one get elected to be the leader of our great nation? Well don’t worry the constitution has rules regarding that as well. The constitution has three requirements to become eligible to run for president. One must be thirty-five years of age, be a resident within the United States for fourteen years, and you must be a natural born citizen. With those parameters set, the on ly thing left to do is vote. Electing the president is a carefully planned process that has been used since 1787 by the framers of the Constitution. The way we vote is through what is called the Electoral College. Over time, some states have altered the way they implement using the system, but the fundamentalsShow MoreRelatedA comparison of the United States Constitution And The Declaration of Independence712 Words   |  3 PagesIntroduction The United States Constitution and The Declaration of Independence are two of America s most famous documents that laid the foundation for it s independence as a nation and separation from British rule. The following paper will compare these two documents and decipher the difference of the two. While both Declaration of Independence and the United States Constitution contain important information regarding America s independence they are also different in many respects. DraftedRead MoreThe Constitutional Convention Of The Constitution1179 Words   |  5 PagesMost of the framers of the constitution either attended or graduated college, were involved in the American Revolution, and had already been involved in the government. The Constitutional Convention was a meeting held in Philadelphia between May and September of 1787. There, delegates discussed revisions to the United States Government. The Constitutional Convention was held in order to address the problems of the weak central government that existed under the Articles of Confederation. Many of theRead MoreThe Articles Of Confederation And The United States1375 Words   |  6 Pagesfirst constitution of the United States of America, and the US Constitution, which acts as the supreme law in the United States today, bo th are poles apart. In fact, it was only because of the weaknesses of the Articles that the present-day US Constitution was drafted. The Articles of Confederation or Articles was a written agreement which laid the guidelines for the functioning of the national government. It was drafted by the Continental Congress and sent to the thirteen original states for ratificationRead MoreHow Have the Concepts of Popular Sovereignty, Rule of Law and Tolerance Developed Through Time880 Words   |  4 PagesJOURNAL 1 The United State has been established based on the foundation ideas of Popular Sovereignty, Rule of Law, and Tolerance. Those ideas have become the strong pillars for our Founding Fathers to build a new society (Coaty 23) which vividly accounted in the U.S. Constitution. The questions arise, what are the Popular Sovereignty, Rule of Law, and Tolerance? Why those ideas have become so vital?   The Popular Sovereignty means is a form of power that a nation state has over itself and its peopleRead MoreThe Constitutional Convention Of Philadelphia Signed The United States Constitution1107 Words   |  5 PagesConstitutional Convention in Philadelphia signed the United States Constitution on September 17, 1787. Designed to replace the weak Articles of Confederation previously in place, the Constitution delineates and outlines the powers of the different branches of government. The Constitution also includes the Bill of Rights that states the rights of the citizens. As of this year, the Constitution is two hundred and twenty-nine years old. The Constitution continues to have an impact on modern society as itRead MorePrinciples Of The Nation s Constitution And The Bill Of Rights1413 Words   |  6 Pagesin the nation. The principles are clearly defined in the nation’s constitution and the bill of rights. The important principles give people a sense of national identity of belonging to a certain nation, hold the people together and allow the government to function competently. In American government, there are fundamental principles which have been established and are used in the governing of the people. The principles include rule of law, democracy, representative government, limited government andRead MoreThe Constitutional System Within The United Kingdom1416 Words   |  6 Pagesand power of a government, but also state the relationship between government and individuals. Constitutionalism can be classified into two distinct categories: a codified written Constitutions (ie: United States), and an uncodified unwritten constitution (ie: United Kingdom). Some consider the UK constitution as a risk to the democratic society due to its flexibility and the chance of forming dictatorship. However, others believe that an uncodified constitution could be beneficial within a constantRead MoreThe Many Variations and Common Themes of Constitutions1499 Wor ds   |  6 Pagesfought in the war. This is known as a constitution, which aids to governing and shaping a country. A renowned example of a constitution is the United States constitution, which was written and ratified a few years after the American Revolutionary War. Not only does the U.S. have a constitution, but also France, Iraq, Vietnam, and other nations. Even ancient civilizations established one, such as Greece, Rome, Babylon, the Iroquois Confederacy, and others. Constitutions imply different themes, such as rightsRead MoreGorn Chapter 5 Questions Essay1231 Words   |  5 Pagesawarded to congress in the Constitution of 1787 would an Anti- Federalist be most likely to oppose? Anti- Federalist were against the Constitution all together but most of all, I think the Anti-Federalist were against the fact that the state government’s would no longer have as much power like it did with the Articles of Confederation. The constitution supported the idea of a powerful and strong central government. The congress had the power to tax people, and make rules and regulations as it saysRead More Comparison of US Bill of Rights and The Canadian Charter of Rights1405 Words   |  6 Pages BACKGROUND OF THE BILL OF RIGHTS The United States Bill of Rights came into being as a result of a promise made by the Fathers of Confederation to the states during the struggle for ratification of the Constitution in 1787-88. A great number of the states made as a condition for their ratification, the addition of amendments, which would guarantee citizens protection of their rights against the central government. Thus, we have a rather interesting situation in which the entrenchment of a bill

Thursday, December 12, 2019

Culture of the organization and Change management †Free Samples

Question: Discuss about the Culture of the organization and Change management. Answer: Literature review The concept of the organizational culture is one of the hotly debated topics in organization science. The researchers, managers and public often use the term organization culture, even though there is no clear interpretation universally. All the organization has culture, but some are stronger than others(Deshpande Webster Jr, 1989). Culture is the total sum of the knowledge, beliefs, customs and the attitudes to which people expose themselves in their social condition. Individuals can acquire values; learn a language and habits of the thoughts and behaviors(Carmeli Sheaffer, 2009). Every day in our life we are facing the idea of the change. Changes always affect the workers in the organization and therefore it is important to manage it carefully to avoid the resistance to change or failure of the modification. Change in the management can take in radical form, gradually or rapidly. According to the Lewin's model; the refreezing stage is a good definition of the change (Burnes, 2005). In the 21st century, there is no need to refreeze because competition will overtake the organization. (Zeffane, 1996) suggests that change management is how the change affects the organization, and the processes are the same irrespective of whether there is reassigning of the work practices in one area or the re-specification of the management and whole organization structure Organization is dynamic entities and therefore changes all the time due to the development of their outputs and operation in the case of the effectiveness and efficiency. Management of the change is one of the most discussed topics in the management field(Patterson, Wa rr, West, 2004). Hence, it is crucial to understand why individuals resist change to support that part which cannot cope with the modification through coaching and motivate and handling the problems that arise as a result of the change. As William Shakespeare thought Thing must change or cease (Day, 1994). Management of change is about detailed planning, inspirational leadership and comprehensive implementation. Culture of the organization or group is a collection of beliefs and assumption about the world, nature of the space and time, human relationships and life. The beliefs are cultured responses to the problems of the survival in the external environment(Prajogo McDermott, 2005). The core of the existence of the organization is the fundamental external problems. The primary objectives are based on these strategies and mission to conform to the mentioned purpose. Culture establishes and reinforces order, membership criteria, conditions, communication patterns, priorities, punishment and nature of power and reward, management practices and decision-making processes. Numerous models describe the relationship between the variables and phenomena of organization culture (Khazanchi, Lewis, Boyer, 2007). The system approach emphasizes on the interdependencies between various elements and sub-system in an organization and also offers a holistic approach. The organization system models show the relationship between organization sub-system; technology, psycho-sociology, management, structure and goal(Khazanchi, Lewis, Boyer, 2007). The primary determinant in the workplace depends on the complex interaction between groups and individuals which take place at different levels within the firm, between other body and external environment. Complex environment represents the patterns of the interaction between roles, peoples, and the external technology in business. The dimensions of the culture encompass the following; mission and vision; it determines personnels understanding of the values, vision and purposes of the company and it can transform into team goals and objectives (Spender, 1996). External environment determines the extent of focus on internal customers, external and employees perception of the effectiveness of community surrounding (Spender, 1996). Another dimension is the leaderships which concentrate on particular sections that strengthen leadership. Interpersonal relationship focuses on the relationship between personnel, managers and management of conflict. Management process focuses on the method in which management takes place including formulation of the goals, controls and innovation process, goal development and communication (Prajogo McDermott, 2005). Finally, the organization image focuses on the how the outside perceives the organization. As earlier mentioned, five concepts of the organization culture include; job challenge, communication, trust, innovation and social cohesiveness (Prajogo McDermott, 2005). Job challenge concept refers to the complexity and diversity of the work. Communication principles focus on the effectiveness of communication between employees, top management and between employees(Spender, 1996). Trust exists between the managers and their staff or in-between employees who enable open-minded environment and free discussion. Social cohesion is the relationship among members of the organization which further features as a sense of solidarity and cooperation (Ford, 1999). Finally, innovation concept is the supportive environment for new ideas, problem-solving and creativity. In managing change, consideration of areas such as activities, behaviors and structure is vital. Leadership affects culture more than management(Ford, 1999). For the employee to acceptance, employees need to understand the requirement for the new positions and organization implications regarding the relationships and behaviors and new activities which can result in their needs satisfaction. Successive change comes through the participative approach of the management even though it originates from the senior level of the control(Day, 1994). To cope with change and ensure acceptance and motivation, managers should listen to employee concerns accordingly and provide them with advice, assist and support. (Burnes, 2005) Suggest managing change model that relates to the managerial strategies to counter the resistance to the change. They suggest that contingency approach should adapt to every change in management; the leaders should select the best way of responding to an individual situation. Selection for appropriate management style and strategy for change is crucial for each type of resistance. Basically, six approaches have been put forward as a method of controlling resistance to change; education and communication, participation, negotiation and agreement, facilitation and support, and coercion (Burnes, 2005). Firstly, The McKinsey 7S model comprises of S component and changing of any s it will have an impact on the other elements. This element includes; staff, skills, systems, style, shared values, strategy and structure. The McKinsey 7s model provides an efficient model for analyzing an organization and its operation that determines if the organization is weak to adjust to any changes(Burnes, 2005). Additionally, the model assists in avoiding sources that contribute to resistance to change. Secondly, Lewin three step model of change consists of the steps; unfreezing, changing and refreezing (Burnes, 2005). The unfreezing stage, there is the creation of the motivation for the change in the workforce. At this point, the employee should get enough reasons, and good communication is essential before the implementation step. Hence, to avoid the resistance to change, management should encourage and discuss, and explain the reasons for the change. Change level is where there is an identification of the new behavior, procedure and encouragement of employee to adopt new norms (Patterson, Warr, West, 2004). Managers should implement change successfully, monitors and assess the feeling of workers. Refreezing is the final stage and leader should reinforce, and stabilize the new culture to avoid employee to go back to the old habit (Burnes, 2005). Incentives regarding the rewards are crucial for motivating employees for adapting to the new culture. Hence, the whole process is achie vable through communication, leadership, training and education. Different researchers explain the concept of the change communication in a very differing way. Change communication is the propagation of information that concentrates on the training, knowledge, ideas, facts and directives of action concerning the change(Armenakis Bedeian, 1999). (Todnem By, 2005), argues that communication is informing the desired changes as well as giving the reasons for them. Effective communication helps the leaders to get the individuals in the accomplishing certain task and therefore assist in implementing change successfully. (Todnem By, 2005), proposes that if employees do not participate in the change process, they will feel that there is no value to their opinions and suggestions. Study shows that the participations raise the level of the commitment among workers and decrease the levels of the resistances towards a particular change(Mintzberg Westley, 1992). Participation offers specific benefits to workers to reduce the degree of the resistance of the employees. Conclusion Clearly, in the present days, change is inevitable, and it is vital in the current competition for a business to survive in its operation. Taking the company at the right time and step will save the firm from getting out the business. Change should reflect the organizations goals in a sense that the organization members can understand, implement and its goals accordingly. For the resistance reduction, there is no universal way, but likely approaches suggest in various management literature. Moreover, participative management is one of the better ways to reduce resistance. Additionally, the participative approach is useful in improving the change process. An analysis of the organization culture and on how to overcome resistance to change shows that change will always affect all the organization globally. Therefore, it is safe not to be reactive but proactive. For the organization change to occur efficiently, then innovation is very crucial. It is also essential to manage change carefully and successfully and avoid change resistance since change always affects people in an organization. Structure, people and technology are factors that determine the organizational effectiveness(Todnem By, 2005). Structural are more of policies, control and procedures of the organization. People factors are leadership skills, attitudes and characteristics of the human resources. Lastly, technology factors are any types of the process that helps members in the performance of their jobs. References Armenakis, A., Bedeian, A. (1999). Armenakis, A. A., Organizational change: A review of theory and research in the 1990s. Journal of management, 25(3), 293-315. Burnes, B. (2005). Complexity theories and organizational change. International Journal of Management Reviews, 7(2), 73-90. Carmeli, A., Sheaffer, Z. (2009). How leadership characteristics affect organizational decline and downsizing. Journal of Business Ethics, 86(3), 363-378. Day, G. ( 1994). The capabilities of market-driven organizations. the Journal of Marketing, 37-52. Deshpande, R., Webster Jr, F. (1989). Organizational culture and marketing: defining the research agenda. The journal of marketing, 3-15. Ford, J. (1999). Organizational change as shifting conversations. Journal of Organizational Change Management, 12(6), 480-500. Khazanchi, S., Lewis, M., Boyer, K. (2007). Innovation-supportive culture: The impact of organizational values on process innovation. Journal of operations management, 25(4), 871-884. Mintzberg, H., Westley, F. (1992). Cycles of organizational change. Strategic management journal, 13(S2), 39-59. Patterson, M., Warr, P., West, M. (2004). Organizational climate and company productivity: The role of employee affect and employee level. Journal of Occupational and Organizational Psychology, 77(2), Patterson, M., Warr, P., West, M. (2004). Organizational climate and company productivity: The rol 193-216. Prajogo, D., McDermott, C. (2005). The relationship between total quality management practices and organizational culture. International Journal of Operations Production Management, 25(11), 1101-1122. Spender, J. (1996). Organizational knowledge, learning and memory: three concepts in search of a theory. Spender, J. C. (1996). Organizational knowledge, learning and me Journal of organizational change management, 9(1), 63-78. Todnem By, R. (2005). Organisational change management: A critical review. Journal of change management, 5(4), 369-380. Zeffane, R. (1996). Dynamics of strategic change: critical issues in fostering positive organizational change. Leadership Organization Development Journal, 17(7), 36-43.

Monday, December 9, 2019

Best Practices In Leading Traditionalists -Myassignmenthelp.Com

Question: Discuss About The Best Practices In Leading Traditionalists? Answer: Introduction Management communication can be said as a systematic planning, evaluation and implementation of all channels of communication within an entity. It comprises organization and propagation of new communicative directives within an organization (Wiedmer, 2015). Presently, every organization is aware regarding the conflicts relating to generations in a workplace as they have different preferences for learning methods.The present report deals with discussion relating to issues faced by HRM director of ABC Company relating to managing different generation and the manner of harmonizing the generational divides present in the company. Further, analysis relating to values and attitude accepted by Generation X (1961-1984) has also been presented in detail manner. Lastly, the reports end up with the discussion relating to decision-making preferences for people belonging to Generation X. Communication Media for Generation X (people born between 1961-1984) In simple words, media can be specified as communicative outlets or tools applied for storing and delivering information or data. It is also associated with specialized mass communication business or communication media. Media has to accomplish specified roles in a society which comprises: entertaining and providing a source of imagination; enhancing education and information; to behave as a watchdog for the government, business and other institutions and serving as a public forum for assessing and evaluating important discussion relating to every sector. Generation X is assessed to be the luckiest group of individuals in which major people exist which born in the late 60s but before 80. The people born during this period are of age between 35 -57 years. Presently we work in an organization where a fresh graduate is seen working beside55-year-old colleague in the same project. However, on the contrary, Generation Y (1980-2000) who believe in learning through computer training and keep updated information on their tips through the internet. This group of people work in an ethnic manner in comparison to boomers. The main emphasis of this group is on diversity, honesty, challenges relating to innovation, creative input. Further, it has been also assessed that they are having a rigid and work-centric approach. It has been assessed that Generation X put emphasis on development generally and prefer to learn independently rather than applying computer based training or internet. The group occupies sixty percent of the existing workforce. Gen eration X seems to be possessing an entrepreneurial spirit, an attitude of doing it yourself (Allan, 2015). They emphasise on the career, however, they give equal importance to family time and make effort for good work-life balance. Values and attitude towards work and decision-making The individual of this group is independent, tech-savvy competent and realistic. This generation is more self-managing and values both work and family. Thus, for motivating and encouraging this group of individuals perks and advantages provided to them should be decided in the same accordance. As Generation X believes in independent training rather than computer training; thus they should be made aware regarding the ease of working and efficient results which can be attained through application of computer training. As they believe in marketing good relationship with the organization and give importance to the family as well work following measures can be taken in accordance with their values and attitude in order to encourage them: Xers seek for balance, thus recruit, retain and motivate this group through developing family-friend programs which provided flexible schedules. Develop program which emphasis on telecommunicating and job-sharing. Xers seek feedback, especially from the manager or other senior executives; thus same should be made a practice for appreciating them for their good work; rather than focusing only towards providing incentives to this group. Effort should be made to make discussion on one- to one basis to these employees for developing a positive relationship and foster trust. They are believed to problem solver; thus they should be included while taking a decision relating to issues faced by the organization (Yi and et.al. 2015). They are self-managing in comparison to young age; thus in case they require some changes in working methods or any other area; the same should be accepted to the extent possible. Keeping in mind the below strengths and weaknesses of Generation X, appropriate strategies of motivation can be devised by the management for their retention in the organization as the entity cannot afford to lose an experienced workforce. As per the views of Wiedmer, (2015) traditional approaches that are used to motivate employees of recent generation don't seem to apply to the contemporary generation of employees called Generation X.The following strategies must be used to motivate the X-ers Providing Room to grow Offering employees of Gen X clear with clear statements of goals and simultaneous allowing them with reasonable autonomy for devising the ways to achieve those goals. Building their interest for acquiring modern skills and knowledge can be achieved by providing them ample opportunities to grow on the job. Gen X tends to have a mentality of "work hard, play hard". Opportunities to make choices as this generation is known for becoming familiarized to "fending for themselves," providing options for task selection, challenges, formulating new processes, developing creative conclusions must be offered to them for appropriate results. The management needs to allow the discretion to use their originality and creativity to achieve the set goals. Mentoring-Relationship-oriented and strong mentorships prove to be of great value for Gen X. The management must careful to not micro-manage them or providing them with rigid guidelines for the completion of the projects (Mencl and Lester, 2014). Spending sufficient time with them and offering understandable and frequent feedback on their progress can prove to bring the best in them. Multitasking-Providing the employees of Gen X with one or more task simultaneously for accomplishment can be achieved if the management does not overwhelms them. They are habituated to multitasking due to their experience and are likely to sort out when and what has to be done (Lyons and Kuron, 2014). They look ahead to challenges of such kind where they have to perform several tasks at once. Strength and Weakness of Gen X STRENGTH WEAKNESS It has been assessed by approximately seventy-eight percent of respondents that they are tech-savvy and are able to use social media for leveraging appropriately. They are believed to be the highest revenue generator. Having capability of possessing traits of adaptability. They are renowned for the skills relating to problem-solving; thus play a vital role in resolving the issues faced by an organization. They are able to collaborate in new techniques; however efficient efforts are required for the same. Although they have entrepreneurial thinking; they are having low rank relating to executive presence. In most of the times, they like to move to another job in 3 to 5 years. They do not prefer learning from computer training. Decision-making preferences It has been assessed that generation X belong to children of older boomer; who grew up with family, financial and social insecurity. In accordance with the study of Costanza and Finkelstein, (2015) they belong to the families where both the parents have worked. Thus, they believe in self-managing and have strong technical skills and result focused. They should be made part of decision procedure of operations as these people are known for their problem-solving capability. As this belief in resolving the issues them self; thus they are proven as worth assets for the organization. As this generation prove to be a bridge between both the generations boomers and Millennial.Further, Generation X is also known for concentrating on increasing employability the career ladder. It has been also assessed that as this are having more skills as well as patients in comparison to both other generations; thus it is believed that better work can be expected from them.They are comparatively loyal towar ds professional and not necessarily to the employee (Becton, Walker and Jones?Farmer, 2014). The main approach with which Generation X works comprises following variants: Coined work-life balance in the workplace Take decision after application of vital technical methodologies. Believe in the independent Provide results through exceeding expectations. Conclusion Every organization working in todays scenario is aware of the conflicts in a workplace due to the increasing age diversity. The people from different generations have different preferences of learning methods. Hence, management should choose an appropriate communication mode for motivation and retention of staff, keeping in view their strengths and weakness. Communication media can be said as an outlet used for delivering information or data to a specified group or individual. This media has to be selected with utmost caution in dealing with the people of generation 1961-76. This is because these people seem to be very resistant towards learning new technologies and retaliate anything that comes in between their establish values. They possess self-managing tactic due to which they can be used by the management for serving the organizational purpose. If managed successfully they can prove to be great assets for an organization. References Acar, A.B. 2014. Do intrinsic and extrinsic motivation factors differ for Generation X and Generation Y.International Journal of Business and Social Science.5(5).Pp.12-20. Becton, J.B., Walker, H.J. and Jones?Farmer, A. 2014. Generational differences in workplace behaviour.Journal of Applied Social Psychology.44(3).Pp.175-189. Costanza, D.P. and Finkelstein, L.M. 2015. Generationally based differences in the workplace: Is there a there-there?.Industrial and Organizational Psychology.8(3).Pp.308-323. DeVaney, S.A. 2015. Understanding the millennial generation.Journal of Financial Service Professionals. 69(6).Pp.11-14. Lyons, S. and Kuron, L. 2014. Generational differences in the workplace: A review of the evidence and directions for future research.Journal of Organizational Behavior.35(S1). Mencl, J. and Lester, S.W. 2014. More alike than different: What generations value and how the values affect employee workplace perceptions.Journal of Leadership Organizational Studies.21(3).Pp.257-272. Wiedmer, T. 2015. Generations do differ: Best practices in leading traditionalists, boomers, and generations X, Y, and Z.Delta Kappa Gamma Bulletin.82(1).P.51. Yi, X., Ribbens, B., Fu, L. and Cheng, W. 2015. Variation in career and workplace attitudes by generation, gender, and culture differences in career perceptions in the United States and China.Employee Relations.37(1).Pp.66-82. Allan S.,JULY 21, 2015. Generations in the Workforce Marketplace: Preferences in Rewards, Recognition Incentive. [Online]. Auditing Through https://theirf.org/research/generations-in-the-workforce-marketplacepreferences-in-rewards-recognition-incentives/1427/. [Accessed on 3rd October 2017]. Doug and Polly W., 2014. What to Expect From Gen-X and Millennial Employees. [Online]. Available Through https://www.entrepreneur.com/article/240556 . [Accessed on 3rd October 2017]. NotterJ.,2013. Conflict and Generations in the Workplace. [Online]. Available Through https://www.td.org/Publications/Blogs/Management-Blog/2013/03/Conflict-and-Generations-in-the-Workplace. [Accessed on 3rd October 2017]. Higginbottom, K.,2016. The Challenges of Managing a Multi-Generational Workforce. [Online]. Available Through https://www.forbes.com/sites/karenhigginbottom/2016/03/17/the-challenges-of-managing-a-multi-generational-workforce. [Accessed on 3rd October 2017].

Thursday, December 5, 2019

Breadtalk Economic Analysis free essay sample

There is a great opportunity to be an entrepreneur in this industry because of its low entry barriers. Having this opportunity, enterprising individuals are attracted to give up professional careers and go into business. Today, many FB entrepreneurs have expanded overseas by setting up franchises, joint ventures or corporate-owned outlets (Springs Singapore, 2008). 1. 2Background information on the company BreadTalk Group was founded in 2000, operates its chain of retail outlets selling breads, buns, cakes and pastries through its principal subsidiary, BreadTalk Pte Ltd. BreadTalk has become a unique Singapore brand that has gained international appeal. The Group owns and operates 34 bakery outlets in Singapore as of 31 Dec 2007, as well as several outlets in various countries regionally, including Shanghai and Beijing. It is most famous for its cream-filled buns topped with pork floss, named Flosss (BreadTalk, 2008). In 2003, the Group opened the world-renowned Din Tai Fing restaurants, famous for its its â€Å"xiao long bao† meat dumplings, in Singapore (BreadTalk, 2008). In 2005, BreadTalk enters further into the China market by acquiring Topwin Investment Holding Pte Ltd, which owns and operates 13 food courts under the award-winning brand-name Megabite in the PRC (BreadTalk, 2008). In October 2005, the Group opened Toast Box coffee shop which inherits the style and atmosphere of coffee shops in 60s and 70s (BreadTalk, 2008). 1. 3How the firm creates value As part of Breadtalk’s unique concept, the layout of retail outlets are designed in a way that customers have a clear view of bakery items on display. The outlets’ â€Å"see thru kitchens† allow the chefs to share the preparation of the freshly browned breads with customers and shoppers. The design of retail outlets also gives customers a warm and friendly atmosphere. To provide customers with high quality and freshly baked breads, breads are kept only one day on the shelves, and disposed at the end of business hours. Breads are created to reflect the local taste so that customers can enjoy the combination of western concept with eastern design. To accommodate the customers’ changing taste and individual differences, it constantly introduces new varieties of products. With so many varieties of selection, the promise is that â€Å"Each person will find something in the shop† and this makes the trip to the BreadTalk worthwhile (BreadTalk, 2008). 2. 0 Factors 2. 1 Factors influencing demand The four factors that determine demand for the bread supplied by BreadTalk are population, price of substitute goods, income and taste and preferences. 2. 1. 1 Population Figure 1: Singapore population Source: Data adapted from Statistics Singapore, www. singstat. gov. sg Figure 1. 1: Total revenue from owned and operated bakery outlets around the world Figure 2. 2: Singapore bakery sales Source: Data adapted from ListedCompany, www. ListedCompany. com Population is one of the factors that determine the demand for bread supplied by BreadTalk. As shown in Figure 1, Singapore population has been increasing year on year. With the growing population, there are more people to consume the products and hence, the sales of BreadTalk also increased from 31. 4 millions in 2006 to 39. 1 millions in 2007, as shown in Figure 2. 2. Thus, population growth tends to increase the number of buyers which increase the demand for BreadTalk. The market demand curve shifts to the right from D1 to D2 as shown in Figure 3. As a result, there is a direct relationship between Singapore’s population and demand for BreadTalk. Figure 3: Increase in demand 2. 1. 2 Price of Substitute goods The price of substitute goods is the second factor that affects the demand for BreadTalk. â€Å"Substitute good is a good that competes with another good for consumer purchases. As a result, there is a direct relationship between a price change for one good and the demand for its ‘competitor’ good† (Layton, Robinson Tucker, 2005). There are so many substitutes of bread from puffs to breads from other brands available in the market. If the price of bread from other brands increases, consumers switch to other cheaper brands. As a result, the demand of BreadTalk increases and demand curve shifts to rightward. 2. 1. 3 Income The third factor that affects the demand for bread is consumer’s income. The products from BreadTalk can be categorized as normal good because it is (BreadTalk total brandind solution, n. d), on average, 20 percent more expensive than other bakeries. â€Å"A normal good is any good for which there is a direct relationship between changes in income and its demand† (Layton et al. , 2005). As shown in Table 3, consumer’s income, as represented by GDP, rose from 49,301 in 2006 to 52,994 in 2007. The sales of BreadTalk increased from 31. 4 millions in 2006 to 39. 1 millions in 2007, as shown in Figure 2. 2. This shows that when consumer’s income increases, they spend more money on normal goods. So, the demand for BreadTalk also increases and the demand curve shifts rightward. Table 1: GDP of Singapore YearPer Capita GDP S$US $ 200546,73828,078 200649,30131,028 200752,99435,163 Source: Data extracted from Statistics Singapore, www. singstat. gov. sg 2. 1. 4 Taste and preferences Another factor that affects the demand for BreadTalk is Taste and preferences of consumers. With today’s changing lifestyle of Singapore, people are becoming health consciousness and demand for convenience (Adwan, 2003). With long working hours, people have no time to prepare rice and dishes which is time-consuming. Bread, which provides equivalent balanced diet, comes quite handy when a quick meal is preferred. Hence, bread has become popular for breakfast in families. According to Statistics Singapore Newsletter, in Singapore only 4 percent of populations do not consume the bread. Among the working groups, it is also popular to enjoy bread during lunch. This trend increases the demand for bread and thus, the demand curve shifts rightward. On the other hand, when people tired of eating bread, the demand curve shifts back to the left. Moreover, people nowadays demand both better quality and wider selection of products. The quality of products from BreadTalk is good and they have more than 150 varieties of products to cater consumer’s changing taste. So, the demand for BreadTalk increases. 2. 2 How a change in consumers’ income influences the price Figure 4: Demand and Supply curve Source: Data extracted from Google, www. images. google. com As mentioned in section 2. 1. 3, consumer’s income has been increasing year by year that they have more purchasing power. Thus, the demand for BreadTalk increases and the sales rose 24. 5% from 2006 to 2007 as shown in Figure 2. 2. As a result, the demand curve shifts rightward from D1 to D2, as illustrated in Figure 4. Accordingly, the equilibrium price of P1 and the equilibrium quantity of Q1 also increase to P2 and Q2 respectively. That means BreadTalk still sells more products even though they increase the price. Equilibrium occurs at any price for which the quantity demanded and the quantities supplied are equal† (Layton et al. , 2005). So, changes in consumers’ income have effect on the price that BreadTalk can sell its products. 3. 0 Market structure and how it affects competition 3. 1 Features BreadTalk is considered as monopolistic competitor with the following characteristics: †¢There are many small firms in the market. †¢The products are different. †¢Low barriers of entry and exit. 3. 1. 1 Many small firms In monopolistic competition, the market consists of many small firms with the small market share. Because there are a large numbers of firms, firm must pay attention to the average market price (Parkin, 2005, p. 284). 3. 1. 2 Product Differentiation Product differentiation means that each firm’s products are slightly different from the products of competitors. For example, the taste of â€Å"floss bun† from BreadTalk is slightly different from other brands’ â€Å"floss bun†. â€Å"Product differentiation can be real or imagined, but it does not matter if it is only imagined so long as consumers believe such differences exist† (Layton et al. , 2005, p. 235). For example, consumers (Layton et al. 2005) may think that BreadTalk sells the best breads in town, although breads from other brands have similar quality. This consumer’s perceived differences are a key success for firm to compete with competitors. 3. 1. 3 Low barriers of entry and exit It is easy to enter into monopolistic competitive market because only a small amount of capital is required to start up a business. Imagine there are numerous different brands of bread in the market compared with the available number of different airlines. 3. 2 How firms in monopolistic competition compete with each other Firms in monopolistic competition compete with each other in non-price competition, which involves advertising, packaging, product development and quality and service. 3. 2. 1 Advertising One way to compete in monopolistic competition market is by advertising. The main purpose of advertising is to create brand awareness. A brand name (Parkin, 2005, p. 293) provides information about the quality of a product to consumers. When consumer recognizes the brand name, he or she tends to choose that familiar brand over other brands because there is a perception that the brand has a better quality than others. BreadTalk market their products through the local media. They have also created publicity for the brand through interviews with the local newspapers and various articles about the products have been published in both the English and Chinese local newspapers (BreadTalk, 2008). 3. 2. 2 Design The design is important to attract the customers. The eye-catching design will definitely win the customers’ attention. Good design differentiates firm’s products from that of competitors. BreadTalk’s products are perfectly crafted and designed with colorful artwork and each bread also has a unique special name of its own. . 2. 3 Product development Since there are a lot of similar products from other firms in monopolistic competition market, firm must be innovative, and constantly introduces new products that will provide firm with a competitive edge. â€Å"A firm that introduces a new and differentiated product faces a less elastic demand curve and is able to increase its p rice and earn an economic profit† (Parkin, 2005). BreadTalk’s product development is very excellent that it is constantly introduce new products to cater customer’s changing taste. â€Å"The company introduces approximately 10 items every four months. Its retail outlet presently offers over 150 varieties of breads, buns, pastries and cakes for customers† (BreadTalk, 2008). 3. 2. 4 Quality and Service â€Å"The quality of a product is the physical attributes that make it different from the products of other firms† (Parkin, 2005). The quality is important to create customer loyalty. If the product is reliable and the service is good, customers will be most likely to come back again in the future. To provide customers with a better service, the retail outlets of BreadTalk is designed in a way that it allows customers to shop in a comfortable environment. For the ease of access to the shop, the outlets are opened near public transport system such as the Mass Rapid Transit stations, bus terminals and several shopping malls (BreadTalk 2008). 4. 0 Conclusion The Group has been making profit since started in 2000 by diversifying and expanding business overseas. Even though most of the profits came from Singapore division, the business cannot be expanded any further because Singapore market is small with tiny size of population. For long-term growth, the group should further seek out the opportunities in others countries with the big market to bring in even more profit.

Monday, December 2, 2019

Onion Dna Extraction free essay sample

ANSWERS TO STUDENT QUESTIONS 1. Buffer: A solution that will maintain a constant pH, chelate metal ions and generally maintain an environment that will prevent disruption of the experiment. Precipitate: The solid which is visible when a substance becomes insoluble from a solution. Filter: To separate by passing a mixture through a selective membrane. Emulsify: To physically break up lipid into smaller lipid globules. 2. To maintain an environment in which the DNA will not be degraded and to help separate the DNA from other cell components. Tris maintains a constant pH. EDTA chelates metal ions. NaCl provides Na+ ions that will block negative charge from phosphates on DNA. 3. Negatively charged phosphates on DNA cause molecules to repel each other. The Na+ ions will form an ionic bond with the negatively charged phosphates on the DNA, neutralizing the negative charges and allowing the DNA molecules to come together. 4. Any non-starchy vegetable should work. We will write a custom essay sample on Onion Dna Extraction or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Potatoes would not work well. 5. Answers will vary. 6. DNA in cells will absorb UV light. When it does it can be damaged. If the damage occurs in a cell cycle gene, then growth may become uncontrolled leading to skin cancer. STUDENT PAGE 1 DNA Isolation from Onion INTRODUCTION The process of isolating DNA from a cell is the first step for many laboratory procedures in biotechnology. The scientist must be able to separate the DNA from the unwanted substances of the cell gently enough so that the DNA is not broken up or shredded. The procedure you will be doing is a modification of the Marmur preparation which is used worldwide in biotechnology laboratories. A Filtrate is made of onions treated with salt, distilled water and detergent(SDS). An onion is used because it has a low starch content which allows the DNA to be seen more clearly. The salt shields the negative phosphate end of DNA which allows these ends to come closer so they can precipitate out of a cold alcohol solution. The detergent causes the cell membrane to breakdown by emulsifying the lipids and proteins of the cell and disrupting the polar interactions that hold the cell membrane together. The detergent then forms complexes with these lipids and proteins, causing them to precipitate out of solution. Collectively, the salt solution and detergent are referred to as a lysing buffer. DNA Extraction Detergent/Salt Solution Add 20 ml of detergent to 20 g non-iodized salt and 180 ml of distilled water. The detergent salt solution is used to break down the fat and proteins that make up the cell membrane. The sale causs the phosphate ends of the DNA to come closer together which will make it easier to precipitate out of solution. | Meat Tenderizer Solution Add 5 g of meat tenderizer to 95 ml of distilled water. Enzymes in the meat tenderizer will break down proteins| Ethanol Solution Add 5 ml of distilled water to 95 ml of ethanol (alcohol). Rubbing alcohol can be substituted. Place this solution on ice, the colder the better. Alcohol is used to precipitate the DNA. Because DNA is soluble in water, alcohol (ethanol) causes the DNA to precipitate and come out of the solution. DNA will rise into the alcohol layer. | The process of extracting DNA from a cell is the first step for many laboratory procedures in biotechnology. The scientist must be able to separate DNA from the unwanted substances of the cell gently enough so that the DNA is not broken up. It is both interesting and important to understand the reason for some of the steps in the procedure below. An onion is used because it has a low starch content, which allows the DNA to be seen clearly. The salt shields the negative phosphate ends of DNA, which allows the ends to come closer so the DNA can precipitate out of a cold alcohol solution. The detergent causes the cell membrane to break down by dissolving the lipids and proteins of the cell and disrupting the bonds that hold the cell membrane together. The detergent then forms complexes with these lipids and proteins, causing them to precipitate out of solution. DNA is a nucleic acid located in the cells nucleus. It is found making up the genetic material and is bound to several types of proteins. The nuclear and the cell membranes are a tough protective barrier, made of lipids and proteins, which need to be eliminated in order to release the DNA. It is very important that the directions be followed carefully to ensure good results.